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Please note that all translations are automatically generated. Click here for the English version.
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01:39 min
August 18th, 2023
DOI :
10.3791/200632-v
Transcript
要标记周细胞,将荧光染料 TO-PRO-3 添加到人工脑脊髓液或 ACSF 中。现在在室温下在黑暗环境中将新鲜切片的大鼠脑在充满染料的ACSF中孵育20分钟。接下来,将带有脑切片的尼龙网过滤器转移到六孔板冲洗室中。
让脑切片在腔室中静置 10 分钟。为了标记非重要周细胞,将 TO-PRO-3 标记的脑切片在 37 摄氏度的共轭异凝集素 B4 中在黑暗中孵育 30 分钟。孵育后,用人工脑脊髓液冲洗脑切片 15 分钟。
接下来,将脑切片放入用 5% 二氧化碳和 95%
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