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Please note that all translations are automatically generated. Click here for the English version.
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01:52 min
September 8th, 2023
DOI :
10.3791/200634-v
Transcript
首先,小心地切开牺牲小鼠的皮肤,从头骨的开口开始,一直延伸到额叶区域。使用剪刀,在不损伤软脑膜的情况下,小心翼翼地抬起和取出头骨,收集整个大脑。将整个大脑浸入洗涤缓冲液中,轻轻冲洗以去除表面血液。
将大脑转移到无菌培养皿中,无需切碎。然后,在显微镜下,使用细尖镊子从大脑表面提取软脑膜。使用无菌显微剪刀,将软脑膜组织切成碎片。
使用给定的成分修复消化酶混合物。向片段中加入 10 毫升混合物,并在 37 摄氏度下孵育 15 分钟。轻轻搅拌以从管底部分离碎片。
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