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Please note that all translations are automatically generated. Click here for the English version.
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02:37 min
January 5th, 2024
DOI :
10.3791/200639-v
Transcript
首先,用一把细剪刀在麻醉小鼠的腹部皮肤上做一个切口,从剑突隆起到膀胱。用一把无创伤镊子,沿着皮肤切口夹住腹膜的一半,确保切口和覆盖的肠道之间有足够的间隙。接下来,沿白缺血线纵向打开腹膜。
然后用湿棉尖小心地拉出肠子。然后将 200 至 300 μL 生理盐水滴在肠道上,并探查输尿管下腔静脉或 IVC、主动脉和肾静脉的通路。烧灼左肾静脉和肾下腔静脉汇合处远端的双侧分支。
检查输尿管和脉管系统是否有渗出。为防止脊髓缺血和跛行的潜在并发症,请保持腰椎分支完整。检查侧支,包
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