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01:45 min
September 15th, 2023
DOI :
10.3791/200662-v
Transcript
为了使用遗传编码的线粒体自噬报告基因评估小鼠胰腺β细胞的线粒体自噬,将从野生型和Mt-Keima小鼠中分离的胰岛在37摄氏度下培养过夜。第二天,将100个胰岛加入一个六厘米的培养皿中,该培养皿含有两毫升胰岛培养基,用于本方案中使用的每个实验条件。接下来,将孔眼解离成单个细胞,并用 FluoZin-3-AM 和 DAPI 染色,如之前基于 Mt-Phagy 染料的方法所示。
然后对样品进行流式细胞术分析。调整前向散射或FSC和侧向散射或SSC电压,以在SSCA与FSCA图上实现细
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