Research
Education
Solutions
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Türkçe
JA - 日本語
PL - Polski
RU - Русский
113 Views
•
02:05 min
October 20th, 2023
DOI :
10.3791/200667-v
* These authors contributed equally
Transcript
首先准备分化的内皮细胞、寄生虫和星形胶质细胞,以组装 iBBB 模型。从分化的内皮细胞中吸出细胞培养基后,用PBS洗涤细胞一次,然后向洗涤的细胞中加入蛋白酶胶原酶混合物,并在37摄氏度下解离细胞5分钟。将蛋白酶胶原酶混合物与解离的细胞转移到含有hECSR的锥形管中,保持混合物与hECSR的比例为1:3,然后将细胞以300G离心三分钟并吸出上清液,然后将所得细胞沉淀重悬于hECSR中。
在37摄氏度下用蛋白酶胶原酶混合物解离分化的寄生虫5分钟。在锥形管中加入适量的N 2 B 27
Sign in or start your free trial to access this content
Explore More Videos
From the series
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2025 MyJoVE Corporation. All rights reserved