Research
Education
Solutions
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Türkçe
JA - 日本語
PL - Polski
RU - Русский
67 Views
•
04:39 min
October 6th, 2023
DOI :
10.3791/200683-v
* These authors contributed equally
Transcript
首先,取一只麻醉的小鼠,将一滴1%托吡卡胺和2.5%去氧肾上腺素滴入眼睛以扩张瞳孔。然后在角膜上涂抹眼胶,以防止干燥和麻醉引起的白内障。接下来,在无菌环境中设置具有直射光路的直立手术显微镜以进行视网膜下注射。
要制备 10 微升玻璃注射器,请拆下针头轮毂并连接 33 号钝针。取下金属轮毂盖,将针头牢固地固定在注射器上。用蒸馏水冲洗针头,以测试渗漏和针头通畅性。
然后清空注射器并小心地放在一边。接下来,将麻醉的小鼠放在垫子上,并将治疗眼朝向显微镜的物镜。涂抹0.5%盐
Sign in or start your free trial to access this content
Explore More Videos
From the series
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2025 MyJoVE Corporation. All rights reserved