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Please note that all translations are automatically generated. Click here for the English version.
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05:00 min
February 23rd, 2024
DOI :
10.3791/200688-v
Transcript
首先,在鞘液罐中的六升去离子水中加入 30 克氯化钠,然后摇晃罐以溶解盐。然后将储罐连接到细胞分选仪。打开细胞分选仪,启动流体系统,等待流体平衡。
设置丢弃延迟。要制备酵母提取物储备溶液,将一等分的碳 13 酵母提取物重悬于 7.5 毫升超纯水和 2.5 毫升甲醇中。然后,为了制备提取缓冲液,将 10 微升碳 13 酵母提取物储备液加入 10 毫升乙腈中并充分混合。
向96孔PCR板的每个孔中加入25微升提取缓冲液,并用盖子盖住孔。将板放在细胞分选仪上以收集分选的细
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