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Please note that all translations are automatically generated. Click here for the English version.
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02:49 min
September 15th, 2023
DOI :
10.3791/200689-v
* These authors contributed equally
Transcript
首先,将安乐死的新生小鼠的头部牢固地固定到位。使用微操作剪刀,沿着矢状缝合线打开头皮。然后,用手指将头皮分开并固定在双侧。
用剪刀切断头盖骨,然后将其向前翻转以进入头骨。使用小型骨膜升降机提取大脑,然后用剪刀尖轻轻刮擦大脑,露出颅底。现在,检查颅骨底部的双侧颞骨。
用剪刀沿中线切开颅骨底部。然后,刮掉皮肤并消除任何不必要的骨头。保存颞骨并将其转移到装有新鲜HBSS的35毫米无菌培养皿中。
接下来,使用一对 5 个数字尖镊子从颞骨的岩部消除 bola 和周
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