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Please note that all translations are automatically generated. Click here for the English version.
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02:01 min
September 15th, 2023
DOI :
10.3791/200690-v
* These authors contributed equally
Transcript
首先,从新生小鼠的颞骨中分离听觉上皮,并将其转移到含有0.25%胰蛋白酶的10毫升新鲜DMEM中,将混合物在37摄氏度下孵育12分钟。使用200微升移液器吸头,使用手术显微镜轻轻地将毛细胞与基底层和其他细胞分开。然后再加入 10 毫升培养基以抑制解聚。
通过70微米过滤器过滤悬浮细胞。将滤液收集在干净的 50 毫升管中,并以 300G 离心 5 分钟。使用1000微升移液器吸头,通过轻轻移液将毛细胞重悬于5毫升培养基中。
现在提前将盖玻片放在六孔板的底部。计数细胞并
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