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Please note that all translations are automatically generated. Click here for the English version.
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03:39 min
September 29th, 2023
DOI :
10.3791/200695-v
Transcript
首先,用PBS冲洗24孔板的上腔室。使用涡旋混合器将BEND3细胞与DMEM培养基混合以制成悬浮液。然后,在 24 孔板上腔室的 PET 膜上接种 200 微升 BEND3 细胞悬浮液。
向板的下腔室中加入1, 200微升完全介质,以稳定上下腔室的渗透压。通过用负压移液器从一侧缓慢去除旧介质并在流体交换过程中沿壁添加新介质来更换介质。在测量 TEER 之前,将电阻器、5% 次氯酸钠溶液、75% 乙醇和双蒸水放在超洁净台上。
打开紫外线照射 30 分钟以消除残留的细菌
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