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Please note that all translations are automatically generated. Click here for the English version.
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02:06 min
November 10th, 2023
DOI :
10.3791/200702-v
Transcript
要对 2D 脂肪细胞谱系进行染色,请将 200 μL 渗透溶液从试剂盒添加到板中。接下来,将细胞在 200 微升油红 O 工作溶液中孵育 10 分钟。随后,去除污渍后用200微升蒸馏水洗涤细胞五次。
要对 2D 成骨细胞谱系进行染色,请在每个孔中加入 200 微升 5% 新鲜硝酸银,然后将板置于紫外线下一小时。将细胞在 200 微升 2.5% 硫代硫酸钠中孵育 5 分钟,然后用蒸馏水冲洗。对于 3D 培养物,首先获得沉淀培养物的冷冻切片。
为了染色软骨细胞谱系的细胞
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