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Please note that all translations are automatically generated. Click here for the English version.
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05:25 min
November 10th, 2023
DOI :
10.3791/200704-v
Transcript
首先,将两个新的传真管标记为混合管 1 和 2。按照给定的表格准备细胞和抗体悬浮液进行流式细胞术分析。孵育试管 30 分钟后,用一毫升染色缓冲液洗涤每个试管两次。
在室温下以 200 G 离心涡旋管 10 分钟。然后将沉淀重新悬浮在 500 微升染色缓冲液中。用 200 至 500 μL FBS 涂覆 48 孔板的孔,并保持 1 小时。
然后移液 200 至 500 μL 的 10%MSC 培养基,去除残留的 FBS。为了制备用于单细胞分选的样品,将一毫升FBS移液
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