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Mesenchymal Stem Cell Sample Preparation, Flow Cytometric Analysis, and Cell Sorting

181 Views

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05:25 min

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November 10th, 2023

DOI :

10.3791/200704-v

November 10th, 2023

•

181 Views

Ayona Gupta^1*, Risani Mukhopadhyay^1*, Himanshi Khandelwal¹, Narendra Nala², Uttara Chakraborty¹

¹Manipal Institute of Regenerative Medicine, Bengaluru, Manipal Academy of Higher Education, Manipal, ²HIV-AIDS Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR)

* These authors contributed equally

Transcript

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Mesenchymal Stem Cell

Flow Cytometric Analysis

Sample Preparation

Cell And Antibody Suspension

Staining Buffer

Compensation Matrix

Single Stain Compensation Tubes

Acquisition Dashboard

Gating Strategy

Collection Plate

Single Cell Sort Purity Mask

Collection Device

From the series

Fluorescence-Activated Single-Cell Sorting of Human Mesenchymal Stem Cells

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