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Please note that all translations are automatically generated. Click here for the English version.
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01:52 min
October 13th, 2023
DOI :
10.3791/200734-v
Transcript
首先,用含氧生理盐水灌注外部溶液五分钟。用移液管将10微升解剖溶液转移到培养皿中。现在,使用注射器针头和显微镜仔细解剖麻醉的已建立野生型和敲除型苍蝇的大脑。
用移液管将准备好的大脑转移到装有五毫升外部溶液的记录培养皿中。用 C 锋利的支架固定大脑。接下来,以 20 倍的速度捕获每个大脑的共聚焦图像。
使用 XYT 扫描模式,以额外的 4.5 倍数字扩增识别蘑菇体神经元。将激光激发设置为 488 纳米,激光功率为 16 微瓦,以获得全脑 GCaMP6m 发射。然后将扫
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