Name: Newborn Blood-Speckle Image Analyses
Uploaded: 2023-12-22T12:40:00
Description: Watch this Scientific Journal Video about Newborn Blood-Speckle Image Analyses at JoVE.com

newborn blood-speckle image analyses 

心エコー検査由来の血液斑点イメージング技術の進歩

Intracardiac blood flow patterns play a key role in cardiac development, starting in fetal morphogenesis and continuing throughout the lifespan1. Hemodynamic shear stress plays a pivotal role in the stimulation of cardiac chamber growth and architecture via the activation of specific genes2,3. This occurs at both the intrauterine stage and in the early stages of life, thus highlighting the importance of hemodynamic influence on early cardiac development and the carry-over into adulthood3.
The laws of fluid dynamics state that blood passing along a vessel wall move slower when closest to the wall and faster when in the center of a vessel, where resistance is lower. This phenomenon can be demonstrated in any large vessel with pulse wave Doppler as the typical Doppler velocity time integral envelope4. When blood enters a larger cavity such as the heart, the blood farthest from the endocardial surface continues to increase its velocity relative to the blood closest to that surface and create a rotational body of fluid, known as a vortex. Once created, vortices are self-propelling flow structures that typically draw in surrounding fluid via negative pressure gradients. Thus, a vortex can move a greater volume of blood than an equivalent straight jet of fluid, promoting greater cardiac efficiency4,5.
The literature suggests that the evolutionary purpose of vortices is to conserve kinetic energy, minimize shear stress, and maximize flow efficiency4,5,6. Specifically for the heart, this includes storing hemodynamic energy in a rotary motion, facilitating valve closure, and the propagation of blood flow toward the outflow tract, as seen in Figure&#160;1. Altered intracardiac blood flow patterns are expected in pathological situations such as volume-overloaded states and in cases with artificial valves7,8. Thus, herein lies the true diagnostic potential of vortices as early predictors of cardiovascular outcomes in adults.
Intracardiac hemodynamics have gained increasing interest in the literature in both adult and pediatric populations. Several modalities are available for the qualitative and quantitative assessment of intracardiac hemodynamics and were comprehensively summarised in a recent review, with a specific emphasis on the intracardiac vortex9. One modality with great promise is echocardiography-derived blood speckle imaging (BSI), which offers the ability to noninvasively measure a number of qualitative and quantitative vortex characteristics, described below, at a relatively low cost and with excellent reproducibility10. BSI is currently commercially available using a high-end cardiac ultrasound system with an S12 or S6 MHz probe. The speckle-tracking features are analogous to those used in tissue speckle tracking to study myocardial deformation11,12,13. Since red blood cells tend to move faster and with a higher Doppler frequency than the surrounding tissue, the two signals can be separated by applying a temporal filter. BSI uses a best-match algorithm to quantify the movement of blood speckles directly without using contrast agents. The blood velocity measurements can be visualized as arrows, streamlines, or path lines with or without underlying color Doppler images, and can highlight areas of complex flow10.
BSI has been shown to have good feasibility and accuracy for quantifying intracardiac blood flow patterns, with excellent validity compared to a reference phantom instrument and pulsed-Doppler7,10,11. Whilst still very novel, BSI is a promising clinical tool for the early diagnosis of various cardiac pathophysiologies. The clinical application of vortex imaging has shown promise in newborn infants. Specifically, the behavior of a vortex in the left ventricle (LV) may have long-term implications on cardiac remodeling and predisposition toward heart failure.
The mechanism linking vortices to left ventricular remodeling is still relatively unexplored, but has been recently investigated in our laboratory and is the subject of ongoing work11. This methodology article aims to describe the use of BSI in exploring intracardiac vortices and discuss the practical and clinical uses of vortices in assessing diastolic function in various populations. A secondary aim is to discuss the clinical relevance of BSI and present some of the work previously performed in neonates.

著者スポットライト:心エコー検査由来の血液スペックルイメージングによる新生児心臓診断の進歩 

新生児における高フレームレート心エコー検査による血液スペックルイメージングによる心臓内渦の評価

We're try to explore why preterm babies when they're born preterm and grow into adults have a significant higher risk of heart failure. We think it has to do with an abnormal cardiac development because they were born 10 or sometimes 15 weeks early and where pressure in the heart has changed. We can now capture this kind of changes with looking at intracardiac blood flow patterns with blood speckle imaging.Hopefully we'll be able to select babies very early on in this crucial period of cardiac development and maybe apply some preventive strategies with a future goal to also prevent heart failure when they grow up to be adults. Well, from a practical point of view, the challenges always fall back on image quality and the acquisition of clear cardiac structures in an echo. And what's more, it's additionally challenging because we're dealing with babies and we sometimes have very unsettled patients.So we did two studies with blood speckle imaging, one to look if the technique was actually feasible in this population and the answer was yes, it's very feasible. It's very nice images of intracardiac blood flow patterns in these preterm population. And the second we followed the cohort of 80 preterm infant, and we measured them at day five to seven and then again at discharge.And then we categorized them in the babies who had this cardiac remodeling of prematurity and the ones who did not. And we found out that the babies who had cardiac modeling also had abnormal vortex formations at day seven. The vortexes were way more larger and rounder than in the babies who did not develop cardiac remodeling.This might be a very important clue moving forward in exploring which babies might be amenable to preventive treatments. Well, currently there's limited research available on the use of echo derived blood speckle imaging and neonates. So what we've currently been doing is looking at cardiac development after preterm birth.But what remains to be explored is the association between diastolic dysfunction and intracardiac blood flow, like vortices and vortex exclamation. Echo derived blood speckle imaging is a completely non-invasive tool compared to other technologies like MRI. It's also fairly cheap to use and it's very useful as a bedside tool, meaning it can be brought into the rooms next to patients and minimizing inconvenience to the patients.

Watch this Scientific Journal Video about Newborn Blood-Speckle Image Analyses at JoVE.com

Newborn Blood-Speckle Image Analyses   

新生児の血斑画像解析 

まず、各患者の2つの心周期を生のDICOM形式で外部メディアに保存し、画像処理ソフトウェアを使用してラボステーションに転送して、詳細なオフライン分析を行います。オフラインになったら、最も目立つ渦または主要な渦を特定します。各クリップの心周期全体で形成される独立した完全な楕円形の渦の数を記録します。左心室内の既知のランドマークに対する主渦の位置を測定します。渦の深さを測定するには、距離測定ツールを使用して、渦の目から僧帽弁輪の中央までの垂直距離を測定します。渦横位置については、渦眼と心室中隔の心内膜境界との間の水平距離を測定します。左心室の長さと幅に対する主渦の垂直方向および水平方向の端間距離を測定することにより、渦の形状を取得します。次に、トレース測定ツールを使用して、メイン渦の最も顕著な点から最も外側の渦リングをクリックしてトレースし、渦領域を取得します。渦が最も目立つ心臓フレームを記録してピーク渦形成時間を評価し、患者の1心周期のフレーム総数に対するフレーム数を計算します。渦が最初に現れるフレームから、渦が円環の形成を失うまでのフレームを測定することで、渦の持続時間を評価します。早産児のBSI評価による包括的な心エコー図は、渦領域とLVおよび拡張期寸法との間に強い正の相関を示しました。渦の持続時間とeon ePrime比は逆相関していた。新生児の静脈還流を記述するために、独自のフローパターンを持つ心臓ランドマークを特徴付けるBSIのアプリケーションが検討されました。右心室では、主渦は、肺動脈弁と動脈の直前に最大面積で中隔に沿って転がる時計回りの回転構造として見られます。右心房における下大静脈と上大静脈からの流入が混ざり合うことで主渦が形成される。左心房は、4つの肺静脈の流れが直接混ざり合っていない領域が限られており、渦を捉えるのが難しい場合があります。

Research

JoVE Journal

Medicine

Newborn Blood-Speckle Image Analyses 

Assessing Intracardiac Vortices with High Frame-Rate Echocardiography-Derived Blood Speckle Imaging in Newborns

The left ventricle (LV) has a unique pattern of hemodynamic filling. During diastole, a rotational body or ring of fluid known as a vortex is formed due ...