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Please note that all translations are automatically generated. Click here for the English version.
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01:50 min
October 27th, 2023
DOI :
10.3791/200783-v
Transcript
首先,将麻醉的小鼠仰卧位固定在固定板上。用皮肤胶带固定四肢。用拇指和食指压缩准备好的嘴巴堵嘴的自由端。
将鼠标的前门牙固定在嘴巴堵嘴的两只手臂之间的梯形凹槽中。确保将带有压舌板的手臂放在下颌骨上。检查上颌第一磨牙,确保其没有龋齿、外伤和牙龈生成。
评估周围的牙龈,确保没有发红、肿胀或瘘管。然后,检查对侧牙齿的健康状况。接下来,用牙齿毛刺以 20, 000 RPM 的速度钻上颌第一磨牙的咬合侧。
取下牙釉质。用注射器每三分钟将生理盐水滴在牙齿上,以防止过热
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