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Please note that all translations are automatically generated. Click here for the English version.
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03:39 min
October 27th, 2023
DOI :
10.3791/200792-v
Transcript
要开始使用血清移液管,请从含有 70% 汇合 MDA-MB-231 培养物的 T75 烧瓶中取出生长培养基。在烧瓶中加入三毫升胰蛋白酶,并在 37 度下与 5% 二氧化碳一起孵育三到五分钟,以将细胞从烧瓶中分离出来。然后中和胰蛋白酶,在烧瓶中加入三毫升DMEM。
将细胞悬液收集在离心管中,并以400G旋转四分钟。使用移液器弃去上清液,并将沉淀重悬于两毫升PBS中。使用细胞计数器,确定细胞浓度。
将 8 次 10 转移到第 5 个细胞中,放入 15 毫升管中,并加入
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