Plating CFSE-Labelled Tumor Cells and Co-Culturing CAR-Expressing Jurkats for In Vitro Cytotoxicity Evaluation

To begin using a serological pipette, remove the growth media from the T75 flask containing 70%confluent MDA-MB-231 culture. Add three milliliters of trypsin to the flask and incubate at 37 degrees with 5%carbon dioxide for three to five minutes t