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01:27 min
October 13th, 2023
DOI :
10.3791/200798-v
Transcript
首先从附睾中收集精子,从表达AcroSensE的转基因模型小鼠中分离出来。在含有0.5毫升MW培养基的3.5厘米组织培养皿中进行精子收集,在37摄氏度下进行15分钟的游出程序。然后使用细镊子小心地从游泳收集盘中取出任何附睾组织。
将含有精子的培养基转移到15毫升锥形管中,并使用摆动桶转子在室温下以100G离心悬浮液一分钟。在塑料移液管的帮助下,收集含有精子的MW上清液,同时丢弃任何粗大组织碎片颗粒并将其转移到圆底管中。通过加入预热至37摄氏度的500微升MW培养基,将上清液体积
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