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用于收获中性粒细胞胞外诱捕器（NET）的大鼠中性粒细胞分离的一步法和两步法

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03:01 min

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April 26th, 2024

DOI :

10.3791/200810-v

April 26th, 2024

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136 Views

Xiangfeng Gong^1*, Yutao Sun^1*, Xiaoxin Zhang^2,3, Zhenghua Xiao^1,4, Li He^1,4, Chaoyi Qin^1,4

¹Department of Cardiovascular Surgery, West China Hospital, Sichuan University, ²Department of Integrated Traditional Chinese and Western Medicine, Sichuan Provincial Pancreatitis Centre, West China Hospital, Sichuan University, ³West China-Liverpool Biomedical Research Centre, West China Hospital, Sichuan University, ⁴Cardiovascular Surgery Research Laboratory, West China Hospital, Sichuan University

* These authors contributed equally

Transcript

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Keywords Rat Neutrophil Isolation

Neutrophil Extracellular Traps NETs

Bone Marrow Harvest

Density Gradient Centrifugation

One step Method

Two step Method

Red Blood Cell Lysis Buffer

Percoll Reagent

Trypan Blue Staining

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高效的大鼠骨髓中性粒细胞胞外陷阱分离

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