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Please note that all translations are automatically generated. Click here for the English version.
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02:35 min
April 26th, 2024
DOI :
10.3791/200811-v
Transcript
首先,将分离的大鼠中性粒细胞重悬于无菌10厘米培养皿中的四毫升补充RPMI培养基中。接下来,向中性粒细胞悬浮液中加入四微升PMA以触发NET的形成。将混合物在 37% 二氧化碳下在 5% 的二氧化碳下孵育 3 小时。
对于阴性对照,在中性粒细胞悬浮液中加入 4 至 5 微升 DNASE1 以降解分泌的 NET。然后,加入四毫升HBSS洗涤NET。用每块板的 4 毫升新鲜培养基冲洗板以分离 NET。
现在,将洗涤介质收集在新管中并经常移液,以确保NET完全重新悬浮。将
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