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Please note that all translations are automatically generated. Click here for the English version.
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05:02 min
October 20th, 2023
DOI :
10.3791/200819-v
Transcript
首先,取出被PBS灌注的斩首小鼠的脊髓。将脊髓浸没在冰上的培养皿中的冷DPBS中。使用 18 号针头和装满冷 DPBS 的 10 毫升注射器,使用液压挤压将整个脊髓隔离在层流罩中。
将脊髓转移到培养皿中,将冷DPBS放在冰袋上。使用层流罩内的显微镜,用锋利的镊子小心地去除任何可见的脑膜。然后,将脊髓转移到空的培养皿中,并使用 10 号手术刀刀片将其切成两到三毫米长的部分。
为了酶解离脊髓细胞,将酶混合物 1 和 2 添加到脊髓片中。在培养皿中旋转酶数次,以确保脊髓涂
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