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Please note that all translations are automatically generated. Click here for the English version.
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02:51 min
November 3rd, 2023
DOI :
10.3791/200824-v
Transcript
首先,解离 hESC 衍生的视网膜色素上皮细胞,制备细胞悬液,然后对细胞进行计数。现在,在室温下以 250g 离心细胞三分钟。倒出上清液,然后将细胞沉淀重悬于冻存培养基中。
接下来,将 1 毫升细胞悬液转移到 1.2 毫升低温小瓶中。将低温瓶放入冷冻容器中,并在零下 80 摄氏度下冷冻过夜。将小瓶转移到液氮中进行长期储存。
为了解冻冷冻小瓶,首先将加热到37摄氏度的金属珠中的培养基加热,然后将10毫升加热的培养基预填充到15毫升的管中。现在,从液氮中取出低温小瓶,并
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