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Please note that all translations are automatically generated. Click here for the English version.
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04:51 min
November 17th, 2023
DOI :
10.3791/200831-v
Transcript
首先,将解剖的按蚊头在37摄氏度的CCD缓冲液中在加热块上预热五分钟。将带有蚊子头的管子转移到杂交炉中,在 37 摄氏度下旋转。接下来,将管子的全部内容物转移到解剖手表玻璃中。
用镊子拿起头部或任何分离的触角,并将它们固定在一毫升的预固定剂中。在 nutator 中,在 4 摄氏度下将预固定器中的头部旋转 24 小时。要进行组织解剖,首先,在冰上用一毫升 0.1%PBS-Tween 冲洗头部。
然后将头部转移到解剖表镜中。在解剖显微镜下,用锋利的镊子从头部取出感兴趣
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