Mosquito Olfactory Appendage Fixation and Fluorescent Probe Amplification for In Situ Hybridization of Chemosensory Genes

To begin, preheat the dissected anopheles mosquito heads in CCD buffer at 37 degrees Celsius on a heat block for five minutes. Transfer the tube with the mosquito heads to a hybridization oven for rotation at 37 degrees Celsius. Next, transfer the