Research
Education
Solutions
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Türkçe
JA - 日本語
PL - Polski
RU - Русский
107 Views
•
03:17 min
October 27th, 2023
DOI :
10.3791/200849-v
* These authors contributed equally
Transcript
首先,将 500 μL 温培养凝胶加入 24 孔板中的孔中。快速将从小鼠分离的解剖整颗门牙转移到孔中。旋转板几次以冲洗门牙。
将 400 微升温热培养凝胶加入培养皿中,并将冲洗干净的门牙转移到培养皿中。定向切牙以将颈袢区域定位在培养皿的中心,并调整顶端切牙的倾斜度以达到所需的成像平面。凝胶凝固后,使用一把细镊子,取出感兴趣区域顶部的任何凝胶。
在培养皿边缘加入约150微升温热培养基以覆盖样品。将外植体培养物置于 37 摄氏度以使组织沉降。一小时后,打开显微镜和双光子
Sign in or start your free trial to access this content
Explore More Videos
From the series
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2025 MyJoVE Corporation. All rights reserved