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Please note that all translations are automatically generated. Click here for the English version.
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01:19 min
January 26th, 2024
DOI :
10.3791/200874-v
Transcript
首先,准备 B16 F10 GP 黑色素瘤细胞悬液。在吸出细胞悬液之前,轻弹管壁以将气泡移动到顶部。使用一毫升注射器吸出 100 微升细胞悬浮液,并推动活塞以将顶部气泡移出。
接下来,将鼠标置于仰卧位,露出腹部。用手指按压鼠标的左后肢,使左腹股沟区域的皮肤完全暴露。剃掉小鼠的左下腹部,并用含有75%乙醇的棉花清洁。
然后将针头插入角度保持在大约45度,向上倾斜,将针头插入左大腿上腹股沟区域的皮下组织中,深度为0.5至1厘米。轻轻插入针头。同时,将细胞缓慢注射到皮下组
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