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Please note that all translations are automatically generated. Click here for the English version.
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01:12 min
January 26th, 2024
DOI :
10.3791/200875-v
Transcript
为了进行过继转移,在肿瘤植入后6至8天,当肿瘤可触及时检查荷瘤小鼠。在转移前一天,腹膜内注射4毫克环磷酰胺。在转移当天,使用 200-U 胰岛素注射器吸出 100 微升 P14 细胞悬浮液。
从注射器中去除气泡。接下来,将小鼠放在笼子里,用红外灯照射5至10分钟,以扩大尾静脉。然后使用鼠标固定器将鼠标固定到位。
拉直尾巴并用75%乙醇擦拭。将针头平行于尾静脉插入,然后轻轻拉回柱塞。如果没有血液流入注射器,则缓慢地将细胞悬液推入静脉。
注射完成后,迅速拔出
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