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Please note that all translations are automatically generated. Click here for the English version.
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02:00 min
January 26th, 2024
DOI :
10.3791/200877-v
Transcript
首先使用 100-U 胰岛素注射器吸出 20 微升 B16F10-GP 黑色素瘤细胞悬液。从注射器中去除气泡。接下来,在接受肿瘤切除手术的小鼠中,将细胞悬浮液注射到一个腹股沟淋巴结中。
从淋巴结远端插入针头,然后缓慢移动到淋巴结的中心。观察淋巴结的明显肿胀,表明液体被准确注射。将等体积的PBS注射到另一侧的腹股沟淋巴结中。
使用 3-0 缝合线缝合切口。用聚维酮碘对伤口周围的皮肤进行消毒,然后将小鼠放在干净的笼子中,并使用红外线保持温暖。将鼠标保持在侧卧位并持续监测
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