Isolation and Cryopreservation of Human Peripheral Blood Mononuclear Cells for Bioenergetic Studies

To begin, add eight milliliters of DPBS into a sterile 50 milliliter conical tube. To this, add eight milliliters of blood and use a three milliliter plastic Pasteur pipette to mix the contents well. Add 15 milliliters of lymphocyte isolation medi