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Please note that all translations are automatically generated. Click here for the English version.
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02:46 min
October 20th, 2023
DOI :
10.3791/200888-v
Transcript
首先,将 8 毫升 DPBS 加入无菌 50 毫升锥形管中。为此,加入 8 毫升血液,并使用 3 毫升塑料巴斯德移液管将内容物充分混合。将 15 毫升淋巴细胞分离培养基加入另一个锥形管中。
将管子倾斜 20 到 30 度。然后使用三毫升巴斯德移液管将第一层血液DPBS混合物轻轻涂抹在隔离培养基上。将剩余的混合物小心地铺在管的侧壁上。
慢慢地将管子直立,将剩余的血液分层到血液层上。在室温下将试管在摆动桶中以1000G离心10分钟,并关闭制动器。血液PBMC混合物将分为
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