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02:13 min
December 1st, 2023
DOI :
10.3791/200890-v
Transcript
运行 SCRAP 后,使用指示的脚本命令执行峰值调用。列出包含示例文件夹和适配器文件的目录的完整路径。然后,定义峰检出的标准,包括最小测序读长数,以及识别峰所需的不同测序文库数。
指示峰是否必须由同一家族的 sncRNA 贡献,这与共享种子序列并可与重叠基因靶标结合的 miRNA 相关。然后,指示参考目录的完整路径、MIR 碱基缩写和参考基因组缩写。高峰调用后,运行高峰注解脚本。
提供所得峰的完整路径。床从峰调用到参考目录和所需的物种进行注释。使用 samtools
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