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Please note that all translations are automatically generated. Click here for the English version.
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01:32 min
December 8th, 2023
DOI :
10.3791/200901-v
Transcript
首先,将麻醉的小鼠放在桌子上。在双眼上滴一滴眼部凝胶。将动物放在头部支架中以固定其位置。
将动物旋转 90 度,使接受手术的眼睛面向天花板。确保动物的活力以清除眼睛周围的区域。固定动物的身体,以尽量减少呼吸引起的头部运动。
将一个圆形盖玻片水平放在眼部凝胶顶部的眼睛上。根据需要调整动物的头部以正确定位盖玻片。在盖玻片上加入一滴PBS,然后小心地将动物放在显微镜载物台上。
将显微镜的物镜转塔调整到适当的观察距离。激活激光以诱导角膜神经消融。神经消融完成后,
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