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Please note that all translations are automatically generated. Click here for the English version.
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02:53 min
January 19th, 2024
DOI :
10.3791/200940-v
Transcript
首先,从术后第一天开始计算小鼠的巴索小鼠量表评分。在手术后第 30 天,进行行为测试,包括走秀、足部故障和旋转测试。对于走秀测试,记录鼠标行走 45 厘米的距离,最大运行时间为 8 秒。
要进行足部故障测试,请记录鼠标行走 60 步。对于旋转测试,请注意鼠标以每分钟 20 转的速度旋转的旋转器上掉下来所需的时间。在手术后第31天,小心地去除安乐死小鼠的脊髓。
在受伤部位上方和下方五毫米处剪断切除的脐带,为石蜡包埋做准备。用切片机从造成的脊髓损伤中心准备五个微米的石蜡
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