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Please note that all translations are automatically generated. Click here for the English version.
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04:31 min
October 27th, 2023
DOI :
10.3791/200942-v
Transcript
首先,将 25 毫米玻璃盖滑块放入六孔细胞培养板的孔中。在每个盖玻片的中心放置一滴聚赖氨酸。孵育完成后,用蒸馏水清洗每个盖玻片三次。
然后用无钙盐水清洗盖玻片两次。接下来,将盖子安装在记录室中并用无钙盐水填充。从所需的果蝇遗传杂交中收集游荡的三龄幼虫。
将幼虫转移到盘子中,然后用蒸馏水清洗幼虫三次。将幼虫放入另一个装有750微升冰冷无钙盐水溶液的玻璃解剖皿中。现在将幼虫放在立体显微镜下。
用一把镊子在腹部后部做一个横向切口。用镊子推动下颚,将幼虫翻过来。
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