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01:25 min
February 9th, 2024
DOI :
10.3791/200948-v
Transcript
首先,将解剖和固定的小鼠肾脏放入PBS中。使用脑基质将肾脏切成一毫米厚的部分。将切片转移到 4% 多聚甲醛中,并在 4 摄氏度下固定过夜。
对于光片显微镜检查,将整个肾脏浸没在 4% PFA 中,并在 4 摄氏度下孵育 48 小时。对于组织清除,将肾脏样本收集在 50 毫升离心管中,然后在 37 摄氏度下以 60 RPM 的振荡将它们清除在立方 L 中。间歇性更换立方L,直到达到令人满意的光学透明度。
在PBS中洗涤样品三次,在室温下轻轻摇动,每次一小时。然后将立方
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