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Please note that all translations are automatically generated. Click here for the English version.
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01:30 min
February 16th, 2024
DOI :
10.3791/200955-v
Transcript
首先,将麻醉的小鼠放在加热垫上,并将其固定在立体定位框架中。沿着头皮的中线做一个 8 毫米的切口,然后从该区域去除结缔组织。将 bregma 和 lambda 标志对齐在同一水平,以实现准确的电极放置。
然后为参比电极或接地电极、锚螺钉和有源电极钻孔作为特定坐标。将定制的电极连接到立体定位框架臂上,并确保其垂直于大脑。将电极植入海马体 CA-1 区域。
然后使用强力粘合剂和牙科粘结剂固定电极。植入两个 1.2 毫米的地脚螺钉以防止移动。接下来,将参比电极或接地电极直
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