Mouse Ocular Lens Equatorial Region Whole Mount Preparation and Confocal Imaging to Understand the Lens Structural-Developmental Relationship

Begin the whole mount preparation of the fixed lens by creating immobilization agarose wedges. To do so, take a glass-bottomed dish, and pour about five to six milliliters of molten 2%agarose, in PBS, into it. Wait until the agarose solidifies.