formulation of a gavage decoction using traditional chinese medicine and its administration into mice

骨肉瘤小鼠的草药煎剂制剂和灌胃标准化

Decoction formula is the most commonly used dosage form of the traditional Chinese medicine and the liquid medicinal drugs1,2. The decoction formula of traditional Chinese medicine is processed by decocting the herbs in water after being soaked, followed by filtering to discard the dregs.
Because the decoction formula can be individualized based on clinical necessities, especially in line with the characteristics of evidence-based treatment in traditional Chinese medicine, this dosage form via trans-oral administration has been applied in clinical practice for thousands of years with specific and irreplaceable advantages3,4. The quality of the decoction formula is directly related to the clinical efficacy.&#160;Therefore, the decoction formula must be prepared based on the principles for the standardized decoction methods to ensure the active ingredients are maximally extracted and protected3. Meanwhile, decoction-ready medicines are the main form of raw Chinese herbs used in clinical practice; the quality standards and production methods of prescribed decoction-ready medicines must comply with the provisions of the Chinese Pharmacopoeia2. Moreover, the prescribed decoction-ready medicines must be soaked in cold drinking water for at least 30 min before being decocted in a special pharmaceutical pot by heating5. Studies have shown that most of the active ingredients can be extracted by decocting for two times, and the&#160;Code of Practice for the Administration of Decoction Rooms of Traditional Chinese Medicines in Medical Institutions also prescribes decoction to be done twice4,6,7. If special Chinese herbs, such as one with hard texture or tonics, are prescribed, the decoction time is recommended to be extended. The decoction utensils and storage containers that directly come in contact with the decoction formula, starting from the container in which the prescribed decoction-ready medicines are soaked, should be chemically stable and have lids, such as casseroles, ceramics, glass and stainless steel. However, aluminum and ordinary plastic products should not be used, while iron and other corrosive utensils should be prohibited to avoid possible chemical reactions, which will reduce therapeutic efficacy and even produce harmful effects4,6,7.
Due to the limitations of performing clinical experiments, small animals bearing the human diseases, such as mice, are often used in medical research to explore the therapeutic efficacy and comprehensive mechanisms of different interventions including the decoction formula of traditional Chinese medicine8,9; however, unlike humans, the decoction formulas should be prepared every day, mixed together, and freshly administered 2x a day in clinical practice. Animals are unable to voluntarily take their medications on time with the required dosage, and most of the animal experiments related to traditional Chinese medicine are gavage-based oral administration of the decoction formulas at present. The decoction formulas used for in vivo animal experiments should be prepared in advance, aliquoted, stored at -20 &#176;C and thawed just before use3,4,10. Moreover, substandard animal immobilization and gavage needle manipulation increase the risk of unnecessary injuries and negative emotions. Therefore, as an important method of trans-oral drug delivery, the skilled gavage technique is particularly important to avoid possible esophageal injury and drug spillage, thus ensuring that each model animal receives an equal amount of prescribed herbal medicine to achieve accurate experimental results. Here, we provide a gavage protocol for the decoction formula of traditional Chinese medicine.

作者聚焦：用于增强动物研究的标准化草药汤剂方案

中药中药汤剂在骨肉瘤模型小鼠中的强饲策略

This study provides a detailed and standardized protocol for about precaution preparation under gradual operation that's providing a reference for those in need. Our research ensures that each animal is dosed equally to produce accurate results. Our study aims to maximize the animal protection and improve the reliability of results from studies of about Decoction formulations.

一种基于中药的管饲汤制剂及其在小鼠中的给药

首先，将 52.5 克 hedyotis diffusa 和 31.5 克带刺黄芩草本植物浸泡在水中 30 分钟。用大火将草药加热至沸腾，然后在温和的火上加热 15 至 25 分钟。接下来，用橡皮筋将三层纱布固定在量杯口上。通过纱布过滤汤剂。将过滤后的汤剂倒入容器中。同样，通过将草药在饮用水中煮沸以淹没草药来制作第二种汤剂。煮沸后，将第二种汤剂倒入与第一种汤剂相同的容器中。现在将 42 克人参浸泡在饮用水中，距离草药表面约 3 厘米。将浸泡过的人参在大火上煮沸 60 分钟。然后将第一种汤剂与其他汤剂一起倒入容器中。在饮用水中制作第二种人参汤剂，刚好足以淹没草药。用温和的火加热这种汤剂40分钟，然后将其过滤到汤剂容器中。将过滤后的汤剂在锅中用大火煮沸。然后将火焰降低到温和的火势。继续煮沸汤剂，直到获得所需的浓度。将21毫升浓缩汤剂按照所需剂量移液到试管中，持续一周。将等分试样存放在零下 20 摄氏度的冰箱中。将鼠标放在鼠标笼的表面上。用左手的拇指和食指抓住它的尾巴。然后用右手的拇指、食指和中指握住鼠标的脖子。按住鼠标将头部、颈部和身体对齐成一条直线。将加载的管饲针从角落插入小鼠的嘴里，并将舌头推到上颚上。保持鼠标身体对齐，并沿着食道轻轻地将管饲针推向胃部。将汤剂配方注射到胃中，然后取下针头并将小鼠放回笼子中。施用药物汤剂可减少小鼠骨肉瘤肿瘤体积。

Research

JoVE Journal

Medicine

Formulation of a Gavage Decoction Using Traditional Chinese Medicine and its Administration into Mice

Gavage Strategy for Decoction Formula of Traditional Chinese Medicine in Osteosarcoma Model Mice

Decoction formula is the most commonly used dosage form in traditional Chinese medicine and applied in clinical practice for thousands of years by ...

科研

医学