formulation of a gavage decoction using traditional chinese medicine and its administration into mice

Urteafkogformulering og sondestandardisering hos osteosarkommus

Decoction formula is the most commonly used dosage form of the traditional Chinese medicine and the liquid medicinal drugs1,2. The decoction formula of traditional Chinese medicine is processed by decocting the herbs in water after being soaked, followed by filtering to discard the dregs.
Because the decoction formula can be individualized based on clinical necessities, especially in line with the characteristics of evidence-based treatment in traditional Chinese medicine, this dosage form via trans-oral administration has been applied in clinical practice for thousands of years with specific and irreplaceable advantages3,4. The quality of the decoction formula is directly related to the clinical efficacy.&#160;Therefore, the decoction formula must be prepared based on the principles for the standardized decoction methods to ensure the active ingredients are maximally extracted and protected3. Meanwhile, decoction-ready medicines are the main form of raw Chinese herbs used in clinical practice; the quality standards and production methods of prescribed decoction-ready medicines must comply with the provisions of the Chinese Pharmacopoeia2. Moreover, the prescribed decoction-ready medicines must be soaked in cold drinking water for at least 30 min before being decocted in a special pharmaceutical pot by heating5. Studies have shown that most of the active ingredients can be extracted by decocting for two times, and the&#160;Code of Practice for the Administration of Decoction Rooms of Traditional Chinese Medicines in Medical Institutions also prescribes decoction to be done twice4,6,7. If special Chinese herbs, such as one with hard texture or tonics, are prescribed, the decoction time is recommended to be extended. The decoction utensils and storage containers that directly come in contact with the decoction formula, starting from the container in which the prescribed decoction-ready medicines are soaked, should be chemically stable and have lids, such as casseroles, ceramics, glass and stainless steel. However, aluminum and ordinary plastic products should not be used, while iron and other corrosive utensils should be prohibited to avoid possible chemical reactions, which will reduce therapeutic efficacy and even produce harmful effects4,6,7.
Due to the limitations of performing clinical experiments, small animals bearing the human diseases, such as mice, are often used in medical research to explore the therapeutic efficacy and comprehensive mechanisms of different interventions including the decoction formula of traditional Chinese medicine8,9; however, unlike humans, the decoction formulas should be prepared every day, mixed together, and freshly administered 2x a day in clinical practice. Animals are unable to voluntarily take their medications on time with the required dosage, and most of the animal experiments related to traditional Chinese medicine are gavage-based oral administration of the decoction formulas at present. The decoction formulas used for in vivo animal experiments should be prepared in advance, aliquoted, stored at -20 &#176;C and thawed just before use3,4,10. Moreover, substandard animal immobilization and gavage needle manipulation increase the risk of unnecessary injuries and negative emotions. Therefore, as an important method of trans-oral drug delivery, the skilled gavage technique is particularly important to avoid possible esophageal injury and drug spillage, thus ensuring that each model animal receives an equal amount of prescribed herbal medicine to achieve accurate experimental results. Here, we provide a gavage protocol for the decoction formula of traditional Chinese medicine.

Forfatter Spotlight: Standardiseret urteafkogningsprotokol til forbedrede dyreforsøg

Gavagestrategi for afkogningsformel af traditionel kinesisk medicin i osteosarkommodelmus

This study provides a detailed and standardized protocol for about precaution preparation under gradual operation that's providing a reference for those in need. Our research ensures that each animal is dosed equally to produce accurate results. Our study aims to maximize the animal protection and improve the reliability of results from studies of about Decoction formulations.

Formulering af en sondeafkogning ved hjælp af traditionel kinesisk medicin og dens administration i mus

Til at begynde med, blød 52,5 gram hedyotis diffusa og 31,5 gram af pigget skullcap urt i vand i 30 minutter. Opvarm urterne til kogning over en stærk ild, og opvarm derefter i 15 til 25 minutter over en mild ild. Brug derefter gummibånd til at fastgøre tre lag gasbind over mundingen af en målekop.Filtrer afkogningen gennem gasbindet. Hæld det filtrerede afkog i en beholder. På samme måde skal du lave en anden afkogning ved at koge urterne i drikkevand lige nok til at nedsænke urterne.Efter kogning hældes den anden afkogning i samme beholder som den første. Sug nu 42 gram ginseng i drikkevand omkring tre centimeter over urternes overflade. Kog den gennemblødte ginseng over en stærk ild i 60 minutter.Hæld derefter denne første afkogning i beholderen med de andre afkogninger. Lav en anden ginseng decoction i drikkevand lige nok til at nedsænke urten. Opvarm denne afkogning over en mild ild i 40 minutter og filtrer den i afkogningsbeholderen.Kog den filtrerede afkogning på en stærk ild i en gryde. Sænk derefter flammen til en mild ild. Fortsæt med at koge afkogningen, indtil den ønskede koncentration er opnået.Pipette 21 ml koncentreret afkogning i rør i henhold til den krævede dosis i en uge. Opbevar alikvoterne i køleskab ved minus 20 grader Celsius. Placer en mus på overfladen af et musebur.Tag fat i halen med tommelfingeren og pegefingeren på venstre hånd. Hold derefter musens hals med tommelfingeren, pegefingeren og langfingrene på højre hånd. Hold musen for at justere hoved, hals og krop i en lige linje.Indsæt den indlæste sondenål i musens mund fra hjørnet, og skub tungen mod overkæben. Hold musekroppen i samme justering og skub forsigtigt sondenålen mod maven langs spiserøret. Injicer afkogningsformlen i maven, fjern derefter nålen og slip musen tilbage i buret.Administration af den medicinske afkogning reducerede osteosarkomtumorvolumenet hos mus.