formulation of a gavage decoction using traditional chinese medicine and its administration into mice

Formulazione del decotto di erbe e standardizzazione del gavage nei topi con osteosarcoma

Decoction formula is the most commonly used dosage form of the traditional Chinese medicine and the liquid medicinal drugs1,2. The decoction formula of traditional Chinese medicine is processed by decocting the herbs in water after being soaked, followed by filtering to discard the dregs.
Because the decoction formula can be individualized based on clinical necessities, especially in line with the characteristics of evidence-based treatment in traditional Chinese medicine, this dosage form via trans-oral administration has been applied in clinical practice for thousands of years with specific and irreplaceable advantages3,4. The quality of the decoction formula is directly related to the clinical efficacy.&#160;Therefore, the decoction formula must be prepared based on the principles for the standardized decoction methods to ensure the active ingredients are maximally extracted and protected3. Meanwhile, decoction-ready medicines are the main form of raw Chinese herbs used in clinical practice; the quality standards and production methods of prescribed decoction-ready medicines must comply with the provisions of the Chinese Pharmacopoeia2. Moreover, the prescribed decoction-ready medicines must be soaked in cold drinking water for at least 30 min before being decocted in a special pharmaceutical pot by heating5. Studies have shown that most of the active ingredients can be extracted by decocting for two times, and the&#160;Code of Practice for the Administration of Decoction Rooms of Traditional Chinese Medicines in Medical Institutions also prescribes decoction to be done twice4,6,7. If special Chinese herbs, such as one with hard texture or tonics, are prescribed, the decoction time is recommended to be extended. The decoction utensils and storage containers that directly come in contact with the decoction formula, starting from the container in which the prescribed decoction-ready medicines are soaked, should be chemically stable and have lids, such as casseroles, ceramics, glass and stainless steel. However, aluminum and ordinary plastic products should not be used, while iron and other corrosive utensils should be prohibited to avoid possible chemical reactions, which will reduce therapeutic efficacy and even produce harmful effects4,6,7.
Due to the limitations of performing clinical experiments, small animals bearing the human diseases, such as mice, are often used in medical research to explore the therapeutic efficacy and comprehensive mechanisms of different interventions including the decoction formula of traditional Chinese medicine8,9; however, unlike humans, the decoction formulas should be prepared every day, mixed together, and freshly administered 2x a day in clinical practice. Animals are unable to voluntarily take their medications on time with the required dosage, and most of the animal experiments related to traditional Chinese medicine are gavage-based oral administration of the decoction formulas at present. The decoction formulas used for in vivo animal experiments should be prepared in advance, aliquoted, stored at -20 &#176;C and thawed just before use3,4,10. Moreover, substandard animal immobilization and gavage needle manipulation increase the risk of unnecessary injuries and negative emotions. Therefore, as an important method of trans-oral drug delivery, the skilled gavage technique is particularly important to avoid possible esophageal injury and drug spillage, thus ensuring that each model animal receives an equal amount of prescribed herbal medicine to achieve accurate experimental results. Here, we provide a gavage protocol for the decoction formula of traditional Chinese medicine.

Autore in primo piano: Protocollo standardizzato di decotto a base di erbe per studi avanzati sugli animali

Strategia del sonda gastrica per la formula di decotto della medicina tradizionale cinese nei topi modello di osteosarcoma

This study provides a detailed and standardized protocol for about precaution preparation under gradual operation that's providing a reference for those in need. Our research ensures that each animal is dosed equally to produce accurate results. Our study aims to maximize the animal protection and improve the reliability of results from studies of about Decoction formulations.

Formulazione di un decotto di sonda gastrica utilizzando la medicina tradizionale cinese e la sua somministrazione nei topi

Per iniziare, immergi 52,5 grammi di hedyotis diffusa e 31,5 grammi di erba di scutellaria spinata in acqua per 30 minuti. Scaldare le erbe fino a ebollizione a fuoco forte, quindi riscaldarle per 15-25 minuti a fuoco dolce. Quindi, usa gli elastici per fissare tre strati di garza sulla bocca di un misurino.Filtrare il decotto attraverso la garza. Versare il decotto filtrato in un contenitore. Allo stesso modo, prepara un secondo decotto facendo bollire le erbe in acqua potabile quanto basta per immergere le erbe.Dopo l'ebollizione, versare il secondo decotto nello stesso contenitore del primo. Ora mettete in ammollo 42 grammi di ginseng in acqua potabile a circa tre centimetri sopra la superficie delle erbe. Fate bollire il ginseng ammollato a fuoco vivo per 60 minuti.Quindi versare questo primo decotto nel contenitore con gli altri decotti. Prepara un secondo decotto di ginseng in acqua potabile quanto basta per immergere l'erba. Scaldare questo decotto a fuoco dolce per 40 minuti e filtrarlo nel contenitore del decotto.Far bollire il decotto filtrato a fuoco vivo in una pentola. Quindi abbassa la fiamma a fuoco leggero. Continuare a far bollire il decotto fino ad ottenere la concentrazione desiderata.Pipettare 21 millilitri del decotto concentrato in provette secondo il dosaggio richiesto per una settimana. Conservare le aliquote in frigorifero a meno 20 gradi Celsius. Posiziona un mouse sulla superficie di una gabbia per topi.Afferra la coda con il pollice e l'indice della mano sinistra. Quindi tenere il collo del mouse con il pollice, l'indice e il medio della mano destra. Tieni premuto il mouse per allineare la testa, il collo e il corpo in linea retta.Inserire l'ago caricato nella bocca del topo dall'angolo e spingere la lingua contro la mascella superiore. Mantieni il corpo del topo nello stesso allineamento e spingi delicatamente l'ago del sonda gastrica verso lo stomaco lungo l'esofago. Iniettare la formula del decotto nello stomaco, quindi rimuovere l'ago e rilasciare il topo nella gabbia.La somministrazione del decotto medicinale ha ridotto il volume del tumore dell'osteosarcoma nei topi.

Research

JoVE Journal

Medicine

Formulation of a Gavage Decoction Using Traditional Chinese Medicine and its Administration into Mice

Gavage Strategy for Decoction Formula of Traditional Chinese Medicine in Osteosarcoma Model Mice

Decoction formula is the most commonly used dosage form in traditional Chinese medicine and applied in clinical practice for thousands of years by ...

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Medicina