formulation of a gavage decoction using traditional chinese medicine and its administration into mice

Urteavkokformulering og sondestandardisering hos osteosarkommus

Decoction formula is the most commonly used dosage form of the traditional Chinese medicine and the liquid medicinal drugs1,2. The decoction formula of traditional Chinese medicine is processed by decocting the herbs in water after being soaked, followed by filtering to discard the dregs.
Because the decoction formula can be individualized based on clinical necessities, especially in line with the characteristics of evidence-based treatment in traditional Chinese medicine, this dosage form via trans-oral administration has been applied in clinical practice for thousands of years with specific and irreplaceable advantages3,4. The quality of the decoction formula is directly related to the clinical efficacy.&#160;Therefore, the decoction formula must be prepared based on the principles for the standardized decoction methods to ensure the active ingredients are maximally extracted and protected3. Meanwhile, decoction-ready medicines are the main form of raw Chinese herbs used in clinical practice; the quality standards and production methods of prescribed decoction-ready medicines must comply with the provisions of the Chinese Pharmacopoeia2. Moreover, the prescribed decoction-ready medicines must be soaked in cold drinking water for at least 30 min before being decocted in a special pharmaceutical pot by heating5. Studies have shown that most of the active ingredients can be extracted by decocting for two times, and the&#160;Code of Practice for the Administration of Decoction Rooms of Traditional Chinese Medicines in Medical Institutions also prescribes decoction to be done twice4,6,7. If special Chinese herbs, such as one with hard texture or tonics, are prescribed, the decoction time is recommended to be extended. The decoction utensils and storage containers that directly come in contact with the decoction formula, starting from the container in which the prescribed decoction-ready medicines are soaked, should be chemically stable and have lids, such as casseroles, ceramics, glass and stainless steel. However, aluminum and ordinary plastic products should not be used, while iron and other corrosive utensils should be prohibited to avoid possible chemical reactions, which will reduce therapeutic efficacy and even produce harmful effects4,6,7.
Due to the limitations of performing clinical experiments, small animals bearing the human diseases, such as mice, are often used in medical research to explore the therapeutic efficacy and comprehensive mechanisms of different interventions including the decoction formula of traditional Chinese medicine8,9; however, unlike humans, the decoction formulas should be prepared every day, mixed together, and freshly administered 2x a day in clinical practice. Animals are unable to voluntarily take their medications on time with the required dosage, and most of the animal experiments related to traditional Chinese medicine are gavage-based oral administration of the decoction formulas at present. The decoction formulas used for in vivo animal experiments should be prepared in advance, aliquoted, stored at -20 &#176;C and thawed just before use3,4,10. Moreover, substandard animal immobilization and gavage needle manipulation increase the risk of unnecessary injuries and negative emotions. Therefore, as an important method of trans-oral drug delivery, the skilled gavage technique is particularly important to avoid possible esophageal injury and drug spillage, thus ensuring that each model animal receives an equal amount of prescribed herbal medicine to achieve accurate experimental results. Here, we provide a gavage protocol for the decoction formula of traditional Chinese medicine.

Forfatter i søkelyset: Standardisert urteavkoksprotokoll for forbedrede dyrestudier

Gavage Strategi for Decoction Formel av tradisjonell kinesisk medisin i Osteosarcoma Model Mus

This study provides a detailed and standardized protocol for about precaution preparation under gradual operation that's providing a reference for those in need. Our research ensures that each animal is dosed equally to produce accurate results. Our study aims to maximize the animal protection and improve the reliability of results from studies of about Decoction formulations.

Formulering av en Gavage Decoction ved hjelp av tradisjonell kinesisk medisin og dens administrasjon i mus

For å begynne, suge 52,5 gram hedyotis diffusa og 31,5 gram av pigget skullcap urt i vann i 30 minutter. Varm urtene til å koke over en sterk brann, og varm deretter i 15 til 25 minutter over en mild brann. Bruk deretter gummibånd til å fikse tre lag gasbind over munnen av en målekopp.Filtrer avkoket gjennom gasbindet. Hell det filtrerte avkoket i en beholder. På samme måte gjør du et nytt avkok ved å koke urtene i drikkevann akkurat nok til å senke urtene.Etter koking, hell det andre avkoket i samme beholder som den første. Bløtlegg nå 42 gram ginseng i drikkevann omtrent tre centimeter over urtenes overflate. Kok gjennomvåt ginseng over en sterk brann i 60 minutter.Hell deretter dette første avkoket i beholderen med de andre avkokene. Lag en ny ginsengavkok i drikkevann akkurat nok til å senke urten. Varm dette avkoket over en mild brann i 40 minutter og filtrer det inn i avkokbeholderen.Kok det filtrerte avkoket på en sterk brann i en gryte. Senk deretter flammen til en mild brann. Fortsett å koke avkoket til ønsket konsentrasjon er oppnådd.Pipett 21 ml av konsentrert avkok i rør i henhold til ønsket dose i en uke. Oppbevar alikotene i kjøleskap ved minus 20 grader Celsius. Plasser en mus på overflaten av et musebur.Ta tak i halen med tommelen og pekefingeren på venstre hånd. Hold deretter musehalsen med tommelen, indeksen og midterfingrene på høyre hånd. Hold musen for å justere hodet, nakken og kroppen i en rett linje.Sett den lastede gavage nålen inn i munnen av musen fra hjørnet, og skyv tungen mot overkjeven. Hold musekroppen i samme justering og skyv forsiktig gavage nålen mot magen langs spiserøret. Injiser avkokformelen i magen, fjern deretter nålen og slipp musen tilbake i buret.Administrasjon av medisinsk avkok reduserte osteosarkomtumorvolumet hos mus.