formulation of a gavage decoction using traditional chinese medicine and its administration into mice

Formulación de decocción de hierbas y estandarización de sonda nasogástrica en ratones con osteosarcoma

Decoction formula is the most commonly used dosage form of the traditional Chinese medicine and the liquid medicinal drugs1,2. The decoction formula of traditional Chinese medicine is processed by decocting the herbs in water after being soaked, followed by filtering to discard the dregs.
Because the decoction formula can be individualized based on clinical necessities, especially in line with the characteristics of evidence-based treatment in traditional Chinese medicine, this dosage form via trans-oral administration has been applied in clinical practice for thousands of years with specific and irreplaceable advantages3,4. The quality of the decoction formula is directly related to the clinical efficacy.&#160;Therefore, the decoction formula must be prepared based on the principles for the standardized decoction methods to ensure the active ingredients are maximally extracted and protected3. Meanwhile, decoction-ready medicines are the main form of raw Chinese herbs used in clinical practice; the quality standards and production methods of prescribed decoction-ready medicines must comply with the provisions of the Chinese Pharmacopoeia2. Moreover, the prescribed decoction-ready medicines must be soaked in cold drinking water for at least 30 min before being decocted in a special pharmaceutical pot by heating5. Studies have shown that most of the active ingredients can be extracted by decocting for two times, and the&#160;Code of Practice for the Administration of Decoction Rooms of Traditional Chinese Medicines in Medical Institutions also prescribes decoction to be done twice4,6,7. If special Chinese herbs, such as one with hard texture or tonics, are prescribed, the decoction time is recommended to be extended. The decoction utensils and storage containers that directly come in contact with the decoction formula, starting from the container in which the prescribed decoction-ready medicines are soaked, should be chemically stable and have lids, such as casseroles, ceramics, glass and stainless steel. However, aluminum and ordinary plastic products should not be used, while iron and other corrosive utensils should be prohibited to avoid possible chemical reactions, which will reduce therapeutic efficacy and even produce harmful effects4,6,7.
Due to the limitations of performing clinical experiments, small animals bearing the human diseases, such as mice, are often used in medical research to explore the therapeutic efficacy and comprehensive mechanisms of different interventions including the decoction formula of traditional Chinese medicine8,9; however, unlike humans, the decoction formulas should be prepared every day, mixed together, and freshly administered 2x a day in clinical practice. Animals are unable to voluntarily take their medications on time with the required dosage, and most of the animal experiments related to traditional Chinese medicine are gavage-based oral administration of the decoction formulas at present. The decoction formulas used for in vivo animal experiments should be prepared in advance, aliquoted, stored at -20 &#176;C and thawed just before use3,4,10. Moreover, substandard animal immobilization and gavage needle manipulation increase the risk of unnecessary injuries and negative emotions. Therefore, as an important method of trans-oral drug delivery, the skilled gavage technique is particularly important to avoid possible esophageal injury and drug spillage, thus ensuring that each model animal receives an equal amount of prescribed herbal medicine to achieve accurate experimental results. Here, we provide a gavage protocol for the decoction formula of traditional Chinese medicine.

Autor destacado: Protocolo estandarizado de decocción de hierbas para estudios mejorados en animales

Estrategia de sonda nasogástrica para la fórmula de decocción de la medicina tradicional china en ratones modelo de osteosarcoma

This study provides a detailed and standardized protocol for about precaution preparation under gradual operation that's providing a reference for those in need. Our research ensures that each animal is dosed equally to produce accurate results. Our study aims to maximize the animal protection and improve the reliability of results from studies of about Decoction formulations.

Formulación de una decocción de sonda nasogástrica utilizando la medicina tradicional china y su administración en ratones

Para empezar, remoja 52,5 gramos de hedyotis diffusa y 31,5 gramos de hierba de escutelaria con púas en agua durante 30 minutos. Caliente las hierbas hasta que hiervan a fuego fuerte, luego caliente durante 15 a 25 minutos a fuego suave. A continuación, use bandas elásticas para fijar tres capas de gasa sobre la boca de una taza medidora.Filtra la decocción a través de la gasa. Vierta la decocción filtrada en un recipiente. Del mismo modo, haga una segunda decocción hirviendo las hierbas en agua potable lo suficiente para sumergirlas.Después de hervir, vierta la segunda decocción en el mismo recipiente que la primera. Ahora remoje 42 gramos de ginseng en agua potable a unos tres centímetros sobre la superficie de las hierbas. Hervir el ginseng remojado a fuego fuerte durante 60 minutos.Luego vierta esta primera decocción en el recipiente con las otras decocciones. Haga una segunda decocción de ginseng en agua potable lo suficiente para sumergir la hierba. Calienta esta decocción a fuego suave durante 40 minutos y fíltrala en el recipiente de la decocción.Hervir la decocción filtrada a fuego fuerte en una olla. Luego baje la llama a un fuego suave. Seguir hirviendo la decocción hasta obtener la concentración deseada.Pipetear 21 mililitros de la decocción concentrada en tubos según la dosis requerida durante una semana. Guarde las alícuotas en un refrigerador a menos 20 grados centígrados. Coloca un ratón en la superficie de una jaula para ratones.Agarra su cola con el pulgar y el índice de la mano izquierda. A continuación, sujete el cuello del ratón con los dedos pulgar, índice y medio de la mano derecha. Mantenga presionado el mouse para alinear la cabeza, el cuello y el cuerpo en línea recta.Inserte la aguja de sonda nasogástrica cargada en la boca del ratón desde la esquina y empuje la lengua contra la mandíbula superior. Mantenga el cuerpo del ratón en la misma alineación y empuje suavemente la aguja de sonda nasogástrica hacia el estómago a lo largo del esófago. Inyecte la fórmula de decocción en el estómago, luego retire la aguja y libere al ratón de nuevo en la jaula.La administración de la decocción medicinal redujo el volumen tumoral de osteosarcoma en ratones.

Research

JoVE Journal

Medicine

Formulation of a Gavage Decoction Using Traditional Chinese Medicine and its Administration into Mice

Gavage Strategy for Decoction Formula of Traditional Chinese Medicine in Osteosarcoma Model Mice

Decoction formula is the most commonly used dosage form in traditional Chinese medicine and applied in clinical practice for thousands of years by ...

Investigación

Medicina