formulation of a gavage decoction using traditional chinese medicine and its administration into mice

Örtavkoksformulering och sondstandardisering hos osteosarkommöss

Decoction formula is the most commonly used dosage form of the traditional Chinese medicine and the liquid medicinal drugs1,2. The decoction formula of traditional Chinese medicine is processed by decocting the herbs in water after being soaked, followed by filtering to discard the dregs.
Because the decoction formula can be individualized based on clinical necessities, especially in line with the characteristics of evidence-based treatment in traditional Chinese medicine, this dosage form via trans-oral administration has been applied in clinical practice for thousands of years with specific and irreplaceable advantages3,4. The quality of the decoction formula is directly related to the clinical efficacy.&#160;Therefore, the decoction formula must be prepared based on the principles for the standardized decoction methods to ensure the active ingredients are maximally extracted and protected3. Meanwhile, decoction-ready medicines are the main form of raw Chinese herbs used in clinical practice; the quality standards and production methods of prescribed decoction-ready medicines must comply with the provisions of the Chinese Pharmacopoeia2. Moreover, the prescribed decoction-ready medicines must be soaked in cold drinking water for at least 30 min before being decocted in a special pharmaceutical pot by heating5. Studies have shown that most of the active ingredients can be extracted by decocting for two times, and the&#160;Code of Practice for the Administration of Decoction Rooms of Traditional Chinese Medicines in Medical Institutions also prescribes decoction to be done twice4,6,7. If special Chinese herbs, such as one with hard texture or tonics, are prescribed, the decoction time is recommended to be extended. The decoction utensils and storage containers that directly come in contact with the decoction formula, starting from the container in which the prescribed decoction-ready medicines are soaked, should be chemically stable and have lids, such as casseroles, ceramics, glass and stainless steel. However, aluminum and ordinary plastic products should not be used, while iron and other corrosive utensils should be prohibited to avoid possible chemical reactions, which will reduce therapeutic efficacy and even produce harmful effects4,6,7.
Due to the limitations of performing clinical experiments, small animals bearing the human diseases, such as mice, are often used in medical research to explore the therapeutic efficacy and comprehensive mechanisms of different interventions including the decoction formula of traditional Chinese medicine8,9; however, unlike humans, the decoction formulas should be prepared every day, mixed together, and freshly administered 2x a day in clinical practice. Animals are unable to voluntarily take their medications on time with the required dosage, and most of the animal experiments related to traditional Chinese medicine are gavage-based oral administration of the decoction formulas at present. The decoction formulas used for in vivo animal experiments should be prepared in advance, aliquoted, stored at -20 &#176;C and thawed just before use3,4,10. Moreover, substandard animal immobilization and gavage needle manipulation increase the risk of unnecessary injuries and negative emotions. Therefore, as an important method of trans-oral drug delivery, the skilled gavage technique is particularly important to avoid possible esophageal injury and drug spillage, thus ensuring that each model animal receives an equal amount of prescribed herbal medicine to achieve accurate experimental results. Here, we provide a gavage protocol for the decoction formula of traditional Chinese medicine.

Författare i fokus: Standardiserat örtavkoksprotokoll för förbättrade djurstudier

Sondstrategi för avkoksformel för traditionell kinesisk medicin i osteosarkommodellmöss

This study provides a detailed and standardized protocol for about precaution preparation under gradual operation that's providing a reference for those in need. Our research ensures that each animal is dosed equally to produce accurate results. Our study aims to maximize the animal protection and improve the reliability of results from studies of about Decoction formulations.

Formulering av ett sondavkok med hjälp av traditionell kinesisk medicin och dess administrering i möss

Till att börja med, blötlägg 52,5 gram hedyotis diffusa och 31,5 gram av den hullingförsedda kalottörten i vatten i 30 minuter. Värm örterna till kokning över en stark eld och värm sedan i 15 till 25 minuter över en mild eld. Använd sedan gummiband för att fästa tre lager gasväv över mynningen på en mätbägare.Filtrera avkoket genom gasväven. Häll det filtrerade avkoket i en behållare. På samma sätt kan du göra ett andra avkok genom att koka örterna i dricksvatten precis tillräckligt för att sänka ner örterna.När det andra avkoket kokar häller du det i samma behållare som det första. Blötlägg nu 42 gram ginseng i dricksvatten cirka tre centimeter över örternas yta. Koka den blötlagda ginsengen på stark värme i 60 minuter.Häll sedan det första avkoket i behållaren tillsammans med de andra avkoken. Gör ett andra ginsengavkok i dricksvatten precis tillräckligt för att sänka ner örten. Värm detta avkok över en mild eld i 40 minuter och filtrera det i avkoksbehållaren.Koka det filtrerade avkoket på hög värme i en kastrull. Sänk sedan lågan till en mild eld. Fortsätt koka avkoket tills önskad koncentration erhålls.Pipettera 21 ml av det koncentrerade avkoket i rör enligt önskad dos i en vecka. Förvara alikvoterna i kylskåp vid minus 20 grader Celsius. Placera en mus på ytan av en musbur.Ta tag i svansen med tummen och pekfingret på vänster hand. Håll sedan i mushalsen med tummen, pekfingret och långfingret på höger hand. Håll musen för att rikta in huvudet, nacken och kroppen i en rak linje.Stick in den laddade sondnålen i musens mun från hörnet och tryck tungan mot överkäken. Håll muskroppen i samma inriktning och tryck försiktigt sondnålen mot magen längs matstrupen. Injicera avkoksersättningen i magen, ta sedan bort nålen och släpp tillbaka musen i buren.Administrering av det medicinska avkoket minskade tumörvolymen hos osteosarkom hos möss.