Name: Opstellen van een model voor coloproctitis-colorectale kanker (UC-CRC) ulcerosa om het therapeutische effect van de Chinese geneeskunde te evalueren
Uploaded: 2023-10-13T12:50:00
Description: Watch this Scientific Journal Video about Establishment of an Ulcerative Coloproctitis-Colorectal Cancer UC-CRC Model to Evaluate the Therapeutic Effect of Chinese Medicine at JoVE.com

establishment of an ulcerative coloproctitis-colorectal cancer (uc-crc) model to evaluate the therapeutic effect of chinese medicine

Liujunzi Afkooksel: een effectieve behandeling voor UC-CRC-preventie

Colorectal cancer (CRC) is a common gastrointestinal malignancy, the third most common malignancy, and the second most common cause of death in the world, accounting for 10% of the global cancer incidence and 9.4% of the total cancer-related death1,2. Genetic factors, chronic inflammation, high-fat diet, diabetes, and abnormal intestinal flora are risk factors for CRC3,4. Among them, inflammatory bowel disease, especially ulcerative coloproctitis (UC), is a clear risk factor for CRC5,6. UC-associated CRC (UC-CRC) is a transition process of inflammation, atypical hyperplasia, and cancer based on chronic inflammation of the colorectum, which is different from the typical adenoma-adenocarcinoma development model of CRC7,8. Compared with the general population, the risk of CRC is approximately 10-40 times higher in patients with inflammatory bowel disease9.
Currently, surgery is still the standard treatment for CRC, and depending on the location and stage of the tumor, radiation therapy, systemic drug therapy, or a combination of both are possible10. Although these traditional treatment modalities have made great progress, due to the high heterogeneity and recurrence rate of CRC, the prognosis is poor, and the treatment effect is not ideal11,12. Therefore, early detection, early diagnosis, and comprehensive treatment are key to improving the survival rate of CRC patients, and it is particularly important to pay attention to the transformation of UC to CRC. Over the years, traditional Chinese medicine (TCM) has attracted much attention in the treatment of UC-CRC or chronic gastritis due to its limited side effects and significant efficacy. Based on dialectical treatment, famous Chinese medicine practitioners of various generations have created a large number of classic prescriptions, such as Huangqi Jianzhong decoction13, Sijunzi decoction14, and Sishen pill15.
Liujunzi decoction (LJZD) originated from the works of Yi Xue Zheng Zhuan compiled in the Ming Dynasty and is a classic prescription in TCM16. As shown in Table 1, LJZD consists of six traditional herbs, including Codonopsis pilosula (Franch.) Nannf. (Dangshen), Poria cocos (Schw.) Wolf (Fuling), Atractylodes macrocephala Koidz. (Baizhu), Glycyrrhiza uralensis Fisch. (Gancao), Citrus reticulata Blanco (Chenpi) and Pinellia ternata (Thunb.) Breit (Banxia), which has the effect of replenishing qi and strengthening the spleen, drying dampness, and resolving phlegm. In modern clinical practice, it is often used to treat chronic gastritis, gastric ulcers, and duodenal ulcers. Modern pharmacological research has shown that LJZD and modified LJZD have high application value in the adjuvant treatment of UC and digestive tract cancer17,18,19.
At present, there are many ways to construct UC-CRC mouse models, but the azoxymethane (AOM)/dextran sulfate sodium (DSS) induced mouse model is the most widely used UC-CRC model; the clinical symptoms, morphological, and pathological observations have proved that the model is very similar to human UC-CRC20,21. The basic principle is to first induce carcinogenesis with chemical carcinogen AOM and then continuously expose mice to the inflammatory stimulation environment of DSS to simulate the continuous damage and repair of intestinal mucosal epithelium, thereby constructing a UC-CRC mouse model22. The aim of this study is to establish a mouse model of UC-CRC by intraperitoneal injection of AOM and cyclic stimulation of DSS in the short term and to evaluate the effect of the drug and the molecular mechanism of LJZD on UC-CRC in order to provide a scientific basis for the treatment of UC-CRC.

Auteur in de schijnwerpers: Liujunzi-afkooksel als een traditionele Chinese behandeling voor Coloproctitis-kanker

Vaststelling van een coloproctitis-kankermodel bij muizen en evaluatie van het therapeutisch effect van de Chinese geneeskunde

This study was on the prevention and treatment of coloproctitis cancer with traditional Chinese medicine. We aim to establish a mouse model for coloproctitis cancer and evaluate traditional Chinese medicine indicators. We confirmed that the Chinese medicine Liujunzi decoction is a effective treatment for coloproctitis cancer prevention.In the future, we will work to identify more sensitive biomarkers for colon and rectal cancer and screen Liujunzi decoction for therapeutic targets.

Watch this Scientific Journal Video about Establishment of an Ulcerative Coloproctitis-Colorectal Cancer UC-CRC Model to Evaluate the Therapeutic Effect of Chinese Medicine at JoVE.com

Opstellen van een model voor coloproctitis-colorectale kanker (UC-CRC) ulcerosa om het therapeutische effect van de Chinese geneeskunde te evalueren

Plaats om te beginnen de muis voorzichtig met zijn buik omhoog en het hoofd iets naar beneden. Pak de rughuid vast om de buikstreek vast te zetten en steek een injectienaald van één milliliter in, een centimeter rechts van de middellijn van de buik op het snijpunt van de lijn, die beide dijen verbindt. Steek de injectienaald van één milliliter geleidelijk in tot een diepte van drie tot vijf millimeter onder de huid.Steek de naald ongeveer 0,3 tot 0,5 millimeter in de buikholte in een hoek van 45 graden. Zodra de punt van de naald door de buikspier gaat, merkt u een plotselinge afname van de weerstand op. Injecteer langzaam 0,1 milliliter van de azoxymethaan-injectieoplossing per 10 gram lichaamsgewicht in de muizen.Trek vervolgens de spuit voorzichtig terug. Voeg vervolgens 500 milliliter steriel gedestilleerd water toe aan 10 gram DSS. Om een 2%DSS-oplossing voor te bereiden.Meng de oplossing met een vortexmixer en bewaar bij vier graden Celsius tot gebruik, dien de bereide LJZD en vijf aminosalicylzuuroplossingen dagelijks op een consistent tijdstip via maagsonde toe aan de respectievelijke groepen in week zeven en week 15.

Research

JoVE Journal

Medicine

Establishment of an Ulcerative Coloproctitis-Colorectal Cancer (UC-CRC) Model to Evaluate the Therapeutic Effect of Chinese Medicine

Establishment of Coloproctitis Cancer Model in Mice and Evaluation of Therapeutic Effect of Chinese Medicine

Colorectal cancer (CRC) is a common malignancy of the digestive system and has become the third most common malignancy worldwide and the second leading ...