Name: Valutazione di un modello di coloproctite ulcerosa-cancro del colon-retto (UC-CRC) ed efficacia del decotto di Liujunzi (LJZD)
Uploaded: 2023-10-13T12:50:00
Description: Watch this Scientific Journal Video about Evaluation of an Ulcerative Coloproctitis-Colorectal Cancer UC-CRC Model and Efficacy of Liujunzi Decoction LJZD at JoVE.com

evaluation of an ulcerative coloproctitis-colorectal cancer (uc-crc) model and efficacy of liujunzi decoction (ljzd)

Decotto Liujunzi: un trattamento efficace per la prevenzione dell'UC-CRC

Colorectal cancer (CRC) is a common gastrointestinal malignancy, the third most common malignancy, and the second most common cause of death in the world, accounting for 10% of the global cancer incidence and 9.4% of the total cancer-related death1,2. Genetic factors, chronic inflammation, high-fat diet, diabetes, and abnormal intestinal flora are risk factors for CRC3,4. Among them, inflammatory bowel disease, especially ulcerative coloproctitis (UC), is a clear risk factor for CRC5,6. UC-associated CRC (UC-CRC) is a transition process of inflammation, atypical hyperplasia, and cancer based on chronic inflammation of the colorectum, which is different from the typical adenoma-adenocarcinoma development model of CRC7,8. Compared with the general population, the risk of CRC is approximately 10-40 times higher in patients with inflammatory bowel disease9.
Currently, surgery is still the standard treatment for CRC, and depending on the location and stage of the tumor, radiation therapy, systemic drug therapy, or a combination of both are possible10. Although these traditional treatment modalities have made great progress, due to the high heterogeneity and recurrence rate of CRC, the prognosis is poor, and the treatment effect is not ideal11,12. Therefore, early detection, early diagnosis, and comprehensive treatment are key to improving the survival rate of CRC patients, and it is particularly important to pay attention to the transformation of UC to CRC. Over the years, traditional Chinese medicine (TCM) has attracted much attention in the treatment of UC-CRC or chronic gastritis due to its limited side effects and significant efficacy. Based on dialectical treatment, famous Chinese medicine practitioners of various generations have created a large number of classic prescriptions, such as Huangqi Jianzhong decoction13, Sijunzi decoction14, and Sishen pill15.
Liujunzi decoction (LJZD) originated from the works of Yi Xue Zheng Zhuan compiled in the Ming Dynasty and is a classic prescription in TCM16. As shown in Table 1, LJZD consists of six traditional herbs, including Codonopsis pilosula (Franch.) Nannf. (Dangshen), Poria cocos (Schw.) Wolf (Fuling), Atractylodes macrocephala Koidz. (Baizhu), Glycyrrhiza uralensis Fisch. (Gancao), Citrus reticulata Blanco (Chenpi) and Pinellia ternata (Thunb.) Breit (Banxia), which has the effect of replenishing qi and strengthening the spleen, drying dampness, and resolving phlegm. In modern clinical practice, it is often used to treat chronic gastritis, gastric ulcers, and duodenal ulcers. Modern pharmacological research has shown that LJZD and modified LJZD have high application value in the adjuvant treatment of UC and digestive tract cancer17,18,19.
At present, there are many ways to construct UC-CRC mouse models, but the azoxymethane (AOM)/dextran sulfate sodium (DSS) induced mouse model is the most widely used UC-CRC model; the clinical symptoms, morphological, and pathological observations have proved that the model is very similar to human UC-CRC20,21. The basic principle is to first induce carcinogenesis with chemical carcinogen AOM and then continuously expose mice to the inflammatory stimulation environment of DSS to simulate the continuous damage and repair of intestinal mucosal epithelium, thereby constructing a UC-CRC mouse model22. The aim of this study is to establish a mouse model of UC-CRC by intraperitoneal injection of AOM and cyclic stimulation of DSS in the short term and to evaluate the effect of the drug and the molecular mechanism of LJZD on UC-CRC in order to provide a scientific basis for the treatment of UC-CRC.

Autore in primo piano: Il decotto di Liujunzi come trattamento tradizionale cinese per il cancro della coloproctite

Creazione di un modello di cancro della coloproctite nei topi e valutazione dell'effetto terapeutico della medicina cinese

This study was on the prevention and treatment of coloproctitis cancer with traditional Chinese medicine. We aim to establish a mouse model for coloproctitis cancer and evaluate traditional Chinese medicine indicators. We confirmed that the Chinese medicine Liujunzi decoction is a effective treatment for coloproctitis cancer prevention.In the future, we will work to identify more sensitive biomarkers for colon and rectal cancer and screen Liujunzi decoction for therapeutic targets.

Watch this Scientific Journal Video about Evaluation of an Ulcerative Coloproctitis-Colorectal Cancer UC-CRC Model and Efficacy of Liujunzi Decoction LJZD at JoVE.com

Valutazione di un modello di coloproctite ulcerosa-cancro del colon-retto (UC-CRC) ed efficacia del decotto di Liujunzi (LJZD)

Per iniziare, stabilire il modello murino UC-CRC e somministrare la soluzione LJZD e 5-ASA preparata ai rispettivi gruppi. Dopo il trattamento farmacologico, monitorare quotidianamente il peso dei topi dall'inizio dell'alimentazione adattativa fino alla fine del trattamento farmacologico. Osservare e documentare diligentemente la consistenza fecale di ogni topo sperimentale, classificandolo come feci normali, molli o diarrea acquosa.Documentare attentamente qualsiasi sanguinamento fecale osservato negli animali da esperimento, classificandolo in nessun sanguinamento, sanguinamento minimo o sangue visibile nelle feci. Per separare il tessuto colorettale, mantenere i topi sottoposti a eutanasia all'interno di un ambiente anatomico criogenico. Fissare i topi in posizione supina.Usando le forbici, taglia i peli addominali inferiori e sterilizza l'area con etanolo. Usando una pinza per palpebre, afferra delicatamente il punto di intersezione tra le due radici della coscia e la linea mediana addominale. Quindi usa le forbici per creare un'incisione trasversale lunga da uno a 1,5 centimetri.Eseguire un'incisione longitudinale lungo la linea mediana dell'addome, partendo dal punto medio dell'incisione trasversale fino al processo xifoideo. Staccare con cautela i tessuti peri-colorettali in direzione dell'ano, separando il colon-retto dal tessuto circostante. Spingere delicatamente da parte la pelle addominale per esporre completamente il colon-retto.Usando una pinza palpebrale, estrarre il colorectum dalla cavità addominale e sezionarlo dall'ano al cieco. Conservare i tessuti colorettali raccolti in soluzione fisiologica a quattro gradi Celsius. Il gruppo modello UC-CRC ha avuto una significativa perdita di peso rispetto al gruppo di controllo, che è stata alleviata dal trattamento con LJZD.Il trattamento con LJZD ha migliorato l'indice di attività della malattia rispetto al gruppo modello UC-CRC.

Research

JoVE Journal

Medicine

Evaluation of an Ulcerative Coloproctitis-Colorectal Cancer (UC-CRC) Model and Efficacy of Liujunzi Decoction (LJZD)

Establishment of Coloproctitis Cancer Model in Mice and Evaluation of Therapeutic Effect of Chinese Medicine

Colorectal cancer (CRC) is a common malignancy of the digestive system and has become the third most common malignancy worldwide and the second leading ...