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Please note that all translations are automatically generated. Click here for the English version.
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01:01 min
October 20th, 2023
DOI :
10.3791/200983-v
Transcript
首先,在PDA板上铺上10微升木霉的母体悬浮液。在黑暗中将培养物在 28 摄氏度下孵育 7 至 10 天,直到观察到分生孢子并且培养物变绿以获得新鲜的 F1 世代。通过在培养皿中加入三到五毫升 PBS 来洗涤培养物,然后收集并再次将 PBS 分配到培养物上以逐渐去除分生孢子。
重复此过程,直到悬架变为深绿色。将回收的悬浮液从板转移到无菌的15毫升管中。在12摄氏度下以1,160G离心悬浮液五分钟,并将沉淀重悬于5毫升PBS中。
将最终沉淀重悬于两到三毫升PBS中,并
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