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Please note that all translations are automatically generated. Click here for the English version.
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02:11 min
February 9th, 2024
DOI :
10.3791/200987-v
Transcript
用冰冷的PBS洗涤上述处理过的细胞以除去任何残留的培养基,并加入50微升含有PMSF的RIPA裂解缓冲液以裂解细胞。将细胞收集在试管中。将细胞在冰上孵育几分钟以确保完全裂解,然后在12,000g的4摄氏度下离心15分钟。
然后将细胞裂解物与上样缓冲液混合,并在水浴中以 95 至 100 摄氏度加热混合物 5 至 10 分钟以使蛋白质变性。将每个样品中的 10 μL 总蛋白上样到 SDS0-PAGE 凝胶上。在80伏的恒定电压下运行凝胶。
当溴酚蓝到达分离凝胶底部时停
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