Research
Education
Solutions
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Türkçe
JA - 日本語
PL - Polski
RU - Русский
85 Views
•
02:22 min
May 17th, 2024
DOI :
10.3791/200996-v
Transcript
首先,将无菌盖玻片放入六孔组织培养皿的孔中,并将足够体积的聚-L-赖氨酸和层压溶液倒入孔中以覆盖盖玻片。接下来,用保鲜膜密封板以减少蒸发,并在 4 摄氏度下孵育过夜。第二天早上,用无菌PBS清洗盖玻片三次。
将两毫升细胞悬浮液和生长培养基加入培养基中,将10,000个细胞培养到每个含有盖玻片的孔中。用PBS洗涤盖玻片后,用4%多聚甲醛溶液和PBS固定细胞18分钟。在免疫染色之前,用PBS冲洗盖玻片三次,持续五分钟。
将免疫染色和洗涤的盖玻片在 1 至 5 μg Ho
Sign in or start your free trial to access this content
Explore More Videos
From the series
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2025 MyJoVE Corporation. All rights reserved