inducing freezing injury to establish an orofacial muscle fibrosis mouse model

Etablering av en orofacial muskelfibrosmodell hos möss via en frysskada

Orofacial muscles are critical in daily physiological activities such as mastication, speech, respiration, and facial expression1. In congenital orofacial deformities, however, these muscles exhibit atrophic and fibrotic alterations, leading to impaired body health and social cognition2. Facial reconstructive surgery remains the first-line treatment, but up to 30-70% of postoperative patients still suffer from muscle loss and muscle dysfunction3,4 The failure of orofacial muscle regeneration has been attributed to intrinsic factors, which cannot be corrected by surgery alone. 
The emergence of orofacial muscles is an evolutionary novelty, accompanying the complex vertebrate head and chambered heart5,6. Unlike their somite-derived limb counterparts, orofacial muscles originate from the branchial arch7. These phylogenetic and ontogenetical characters may predispose them to distinct regenerative behaviors8. It has been reported that the masseter (MAS) muscle developed severe fibrosis at the time when the tibialis anterior (TA) muscle fully regenerated after exposure to the same extent of injury1,9. However, the underlying mechanism of regeneration remains poorly understood. 
In this study, a freezing injury model of the mice masseter muscle was established to facilitate the investigation into orofacial muscle regeneration. We chose 14 days after injury as the time point for assessing fibrosis phenotype as it was the earliest time point where discernible divergence was detectable between two muscles. Complete regeneration of the MAS after injury requires at least 40 weeks1. Consistently, this study revealed a remarkable deposition of collagen following freezing injury of MAS compared to the regular regeneration of the TA at 14 days post injury. With the help of this model, further mechanistic studies of muscle atrophy and fibrosis can be carried out, which will in turn help the development of potential therapeutic avenues to promote orofacial muscle regeneration after surgery.

Författare Spotlight: Utforska orofacial muskelregenerering – insikter och innovationer

Frysskada i mustuggmuskeln för att fastställa en orofacial muskelfibrosmodell

Current skeletal muscle regeneration studies merely focus on the limb and the trunk muscle, leaving the characteristics and mechanism of orofacial muscle regeneration largely unexplored. The scope of this research is to create and easier and reliable mouse model to facilitate further investigation into muscle regeneration in the orofacial area. Recent developments review that orofacial muscles constitute a unique subset of skeletal muscle with a distinct evolutionary trajectory and developmental origin.They also exhibit different regeneration performances compared with their limb counterparts. A deeper investigation into the orofacial muscle regeneration is warranted to figure out the mechanism underlying these marked differences. The study present a rapid and reproducible method to study orofacial muscle regeneration.The half-freezing injury process took 20 minutes, and the harvest process took only 10 minutes for each mouse, and the mice can tolerate the injury well without any impairment of locomotion. Since our model allows for subsequent comparison between the fibrotic masseter muscle and the fully regenerated tibialis anterior muscle from the aspects of morphology, histology, and molecular regulation, it serves us a power for two for us to delve deeper into the underlying mechanism. Our future research will focus on the causal effects among the alterations of the resident muscle stem cells and multiple niche cells, and correspondingly developed interventions to the process of orofacial muscle fibrosis.

Inducering av frysskada för att etablera en orofacial muskelfibrosmusmodell

Börja med att placera den bedövade musen på operationsbordet. Använd en bomullspinne och applicera hårborttagningspastan på huden och täck tuggmuskeln och tibialis anteriormuskeln. Efter att ha väntat i en till två minuter, tvätta bort pastan med en kirurgisk torkduk.För att exponera tuggmuskeln, palpera först längs den nedre kanten av underkäken. Fem millimeter från kanten skär du upp huden längs linjen och förbinder den orala kommissuren med tragus. För att utföra frysning, använd förkyld pincett för att hålla en bit torris.Placera torrisen längs de långa axlarna på tuggmusklerna direkt på muskelns yta och håll kvar i fem sekunder. Bekräfta att muskeln verkar stel och blekvit omedelbart efter att torrisen tagits bort och sedan återgår till sin normala färg och struktur inom 22 till 25 sekunder. När muskeln har återhämtat sig helt, stäng hudsåret med minst tre 7-0-suturer.För tibialis anterior muskel, palpera längs vaden för att lokalisera den främre kanten av skenbenet. Två millimeter bakom kanten skär du upp huden, med början från knäet och slutar vid fotleden. För att utföra frysning, använd förkyld pincett för att hålla en bit torris.Placera torrisen längs de långa axlarna av tibialis anterior-musklerna direkt på ytan och håll i fem sekunder. Bekräfta att muskeln verkar stel och blekvit omedelbart efter att torrisen tagits bort och sedan återgår till sin normala färg och struktur inom 22 till 25 sekunder. Efter att ha suturerat hudsåret, placera musen på en värmeplatta som hålls vid cirka 38 grader Celsius.Övervaka kontinuerligt tills musen återfår sin rätningsreflex.

inducing-freezing-injury-to-establish-an-orofacial-muscle-fibrosis

Research

JoVE Journal

Biology

Inducing Freezing Injury to Establish an Orofacial Muscle Fibrosis Mouse Model

Freezing Injury in Mouse Masseter Muscle to Establish an Orofacial Muscle Fibrosis Model

Orofacial muscle constitutes a subset of skeletal muscle tissue, with a distinct evolutionary trajectory and development origin. Unlike the somite-derived ...